sheep anti cd169 primary antibody  (R&D Systems)

Journal: Wellcome Open Research

Article Title: Capturing the systemic immune signature of a norovirus infection: an n-of-1 case study within a clinical trial

doi: 10.12688/wellcomeopenres.11300.3

Figure Lengend Snippet: ( A ) IL-2 levels (IU/ml) (black circles), ( B ) IL-12p70 levels (pg/ml) (orange circles), ( C ) the percentage change in TNF-α levels from baseline (red circles), ( D ) IL-6 (violet circles), ( E ) IL-10 (blue circles), ( F ) IFN-γ (pink circles), ( G ) IP-10 (purple circles), ( H ) CRP (brown circles) and ( I ) soluble SIGLEC-1 levels (grey circles) were measured in the norovirus-infected participant versus uninfected participants (green squares +/- SEM). Analytes were measured in serum or plasma. ( B – H ) Data were normalised to and expressed as percentage change from baseline (day 0). The shaded area indicates the period of reported gastroenteritis.

Article Snippet: Detection was performed using a biotinylated sheep polyclonal anti-SIGLEC-1 (R&D Systems) diluted to a final concentration of 200 ng/ml in PBS + 10% FBS and a Europium-Streptavidin detection solution (PerkinElmer), diluted in standard DELFIA buffer.

Techniques: Infection

Journal: Wellcome Open Research

Article Title: Capturing the systemic immune signature of a norovirus infection: an n-of-1 case study within a clinical trial

doi: 10.12688/wellcomeopenres.11300.3

Figure Lengend Snippet: Percentage change in CD40 expression (mean fluorescent intensity, [MFI]) from baseline was determined on ( A ) monocytes (CD14 ++ ), ( B ) immature myeloid DC (CD11c + CD1c − lin − CD123 int CD304 − ) or ( C ) myeloid dendritic cells (CD1c + lin − CD123 − CD304 − ) from uninfected participants (filled squares +/- SEM) or the norovirus-infected participant (filled circles). Data were normalised to and expressed as percentage change from day 0 levels. The shaded area indicates the period of reported gastroenteritis. Inset histograms show CD40 expression at pre-dosing (filled grey) or day 3 (red line) in the norovirus-infected participant for each cell subset. ( D ) Percentage change in HLA-DR expression (MFI) from baseline on monocytes and ( E ) immature myeloid DCs from uninfected participants (filled squares +/- SEM) or the norovirus-infected participant (filled circles). Inset histograms show HLA-DR expression for each cell type at day 3 (blue line) post-IL-2 injection compared to pre-dosing levels (filled grey) in the norovirus-infected participant. ( F ) SIGLEC-1 expression (MFI) was determined on peripheral blood monocytes (CD14 ++ CD16 lo ) in uninfected participants (filled green squares) versus the norovirus-infected participant (filled black circles). The shaded area indicates the period of reported gastroenteritis. All analyses were performed using cryopreserved PBMCs.

Article Snippet: Detection was performed using a biotinylated sheep polyclonal anti-SIGLEC-1 (R&D Systems) diluted to a final concentration of 200 ng/ml in PBS + 10% FBS and a Europium-Streptavidin detection solution (PerkinElmer), diluted in standard DELFIA buffer.

Techniques: Expressing, Infection, Injection